Coding

Part:BBa_K4214001:Design

Designed by: Jol Tekoniemi   Group: iGEM22_Stockholm   (2022-10-07)


Gastric Intrinsic Factor with signal peptide and polyhistidine tag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 21
    Illegal SpeI site found at 94
    Illegal PstI site found at 1316
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 94
    Illegal PstI site found at 1316
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 21
    Illegal SpeI site found at 94
    Illegal PstI site found at 1316
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 21
    Illegal SpeI site found at 94
    Illegal PstI site found at 1316
    Illegal NgoMIV site found at 14
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 353
    Illegal BsaI.rc site found at 1033
    Illegal SapI site found at 1023


Design Notes

We incorporated a His-tag and cloning sites


Source

Human genome

References